HGF/c-Met Axis: A Promising Therapeutic Target and Biomarker for Cancer

Sino Biological has developed a panel of high activity c-MET proteins and HGF proteins [Live Science](   Wednesday 20 October 2021 [View this email online](   SPONSORED CONTENT [HGF/c-MET Axis: Key Factor Responsible for Cancer Therapy]( Mesenchymal-epithelial transition factor ([MET]( also known as c-Met or Hepatocyte growth factor receptor (HGFR), is a [receptor tyrosine kinase]( (RTK) that is overexpressed or mutated in various malignancies. Aberrantly active MET triggers tumor growth, angiogenesis, and cancer metastasis. MET activity is also critical to embryogenesis. [HGF]( also known as scatter factor (SF), is the only known ligand of MET. Upon activation, MET engages with a variety of signal transducers at its intracellular multisubstrate docking site to stimulate the [Ras/MAPK]( [JAK/STAT]( [SRC]( [PI3K/AKT]( [Wnt/β-catenin]( and other signaling pathways. MET and its associated signaling pathways are clinically important therapeutic targets. A number of small-molecule inhibitors and antibodies targeting MET and HGF are being developed as promising therapeutics for cancer, heart disease, autism, and other diseases. Sino Biological has developed high-quality reagents to support the study of MET signaling. c-MET and HGF Proteins Sino Biological has developed a panel of high activity [c-MET proteins]( and [HGF proteins]( from various species to help study the biology of MET signaling. [Human c-MET Protein | 10692-H20B1]( [Cynomolgus c-MET Protein | 90304-C08H]( [10692-H20B1]( Purity: > 90% as determined by SDS-PAGE Activity: • The specific activity was determined to be 10 nmol/min/mg using MBP as substrate. • Binding ability with HGF measured by functional ELISA. [90304-C08H]( Binding ability measured by ELISA. Immobilized human HGF (Cat: [10463-HNAS]( at 10 µg/mL (100 µL/well) can bind canine c-MET. The EC50 is 7 ng/mL. [Human HGF Protein | 10463-HNAS]( [Cynomolgus HGF Protein | 90286-CNAH]( [10463-HNAS]( [90286-CNAH]( Ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 is typically 0.5-5 ng/mL. Ability to inhibit TGFβ1 activity on Mv-1-lu mink lung epithelial cells. The ED50 is typically 2-10 ng/mL. • Recommended c-MET and HGF Proteins Please visit [#Sino Biological]( to search for these products. Catalog # Species Molecule Tag Expressed Host Activity 10692-H20B1 Human c-MET N-GST & His Insect Cells Active 50622-M02H Mouse c-MET C-human IgG1-Fc HEK293 Cells Active 50622-M08H Mouse c-MET C-His HEK293 Cells Active 70008-D08H Canine c-MET C-His HEK293 Cells Active 80004-R02H Rat c-MET C-human IgG1-Fc HEK293 Cells Active 90304-C02H Cynomolgus, Rhesus c-MET C-human IgG1-Fc HEK293 Cells Active 90304-C08H Cynomolgus, Rhesus c-MET C-His HEK293 Cells Active 90304-CCCH Cynomolgus, Rhesus c-MET C-cleavage HEK293 Cells Active 10463-HNAS Human HGF Native CHO Cells Active 50038-M08H Mouse HGF C-His HEK293 Cells Active 50038-MNAH Mouse HGF Native HEK293 Cells Active 70063-DNAH Canine HGF Native HEK293 Cells 80429-RNAH Rat HGF Native HEK293 Cells Active 90286-CNAH Cynomolgus HGF Native HEK293 Cells Active Anti-c-MET and Anti-HGF Antibodies Sino Biological has developed a comprehensive panel of antibodies targeting MET and HGF, which can be used in ELISA, FCM, IHC, WB, as well as neutralization. ELISA kits and ELISA pair sets are also available. [Cell Proliferation was Inhibited by Human HGF Antibody | 10463-mh010]( [Flow Cytometric Analysis of Human c-Met Expression on HepG2 Cells | 10692-R243]( [10463-mh010]( [10692-R243]( Proliferation of U87 MG cells elicited by autocrine HGF was inhibited by increasing concentrations of Human HGF Neutralizing Monoclonal Antibody. The IC50 is typically 1-4 µg/mL. Cells were stained with purified anti-Human c-Met, second antibody conjugated with FITC. • More Antibodies of c-MET and HGF [Anti-c-MET Antibodies]( [Anti-HGF Antibodies]( [Sino Biological]( Sino Biological US Inc.[Tel:]+1-215-583-7898 [Order:][order_us@sinobiologicalus.com]( Service:][cro_us@sinobiologicalus.com]( Liberty Ridge Drive, Suite 101, Wayne, PA 19087 [Future Logo] © Future US, Inc., 11 W 42nd Street, 15th Floor, New York, NY 10036. This email is intended for {EMAIL} To unsubscribe from Live Science emails or update your email preferences, please [click here](. [Privacy Policy]( | [Cookies Policy]( | [Terms and Conditions](